Table II. Summary of proteins and phosphopeptides identified from soluble leaf extracts by 2D CN-PAGE and MS/MS analysis.
| Spot Identifier and Change in pp2a-b′γa | Full Name | Gene | Score/Coverage | Phosphopeptidesb |
| Defense and stress | ||||
| TGG1 ↑ | Thioglucoside glucohydrolase 1 | AT5G26000 | 2,732/39% | R.GYALG(p)TDAPGR.CK.GFIFGVAS(p)SAYQVEGGR.GR.EYVGDRLPEF(p)SETEAALVK.GK.EKYE(p)TNPALYGELAK.G |
| CA1 ↑ | Carbonic anhydrase1 | AT3G01500 | 1,224/27% | |
| AtGSTF2 ↑ | Glutathione S-transferase F2 | AT4G02520 | 1,028/58% | |
| CSD2 ↑ | Copper/zinc superoxide dismutase | AT2G28190 | 229/32% | |
| Amino acid metabolism and Met cycle | ||||
| OLD3 ↑ | Onset of leaf death 3/O-acetyl-Ser(thiol)lyase | AT4G14880 | 688/40% | |
| SAHH1 ↑ | S-Adenosyl-l-homocysteine hydrolase 1 | AT4G13940 | 1,375/39% | R.LVGV(p)SEETTTGVK.RR.I(p)TIKPQTDR.Wc |
| SAHH2 ↑ | S-Adenosyl-l-homocysteine hydrolase 2 | AT3G23810 | 265/18% | |
| ADK1 ↑ | Adenosine kinase 1 | AT3G09820 | 484/39% | |
| ADK2 ↑ | Adenosine kinase 2 | AT5G03300 | 482/35% | |
| GS2 ↓ | Gln synthetase 2 | AT5G35630 | 694/24% | R.(p)TIEKPVEDPSELPK.W |
| GS1.2 | Gln synthetase 1;2 | AT1G66200 | 143/10% | |
| AtGLN1;1 ↑ | Gln synthetase 1;1 | AT5G37600 | 262/24% | |
| AGT1 | Ala-glyoxylate aminotransferase | AT2G13360 | 499/25% | K.(p)TLLEDVKK.IK.L(p)SQDENHTIK.A |
| Carbon metabolism | ||||
| TKL ↑ | Transketolase | AT3G60750 | 902/30% | K.(p)TVTDKPTLIK.VK.(p)SIITGELPAGWEK.AK.AN(p)SYSVHGAALGEK.ER.HTPEGA(p)TLESDWSAK.F |
| FBA | Fru bisphosphate aldolase | AT4G38970 | 582/30% | K.Y(p)TGEGESEEAK.ER.A(p)TPEQVAAYTLK.LK.DRA(p)TPEQVAAYTLK.LK.RLDSIGLEN(p)TEANR.Q |
| MDH | Malate dehydrogenase | AT1G04410 | 774 | |
| FBP | Fru-1,6-bisphosphatase | AT1G43670 | 681/46% | |
| PRK ↑ | Phosphoribulokinase | AT1G32060 | 1,731/28% | K.FYGEV(p)TQQMLK.H |
| IDH | Cytosolic NADP+-dependent isocitrate dehydrogenase | AT1G65930 | 711/17% | |
| Other | ||||
| CA2 | Carbonic anhydrase 2 | AT5G14740 | 642/50% | |
| CRT1 | Calreticulin 1 | AT1G56340 | 587/33% | |
| Cpn60α ↑ | Chaperonin-60α | AT2G28000 | 785/31% | |
a
The spot identifications are as presented in Figure 4. Protein spots showing higher or lower intensity as compared with the wild type are indicated by arrows.
b
For identification of phosphopeptides, Mascot analysis of MS/MS spectra was conducted by restricting searches for the Arabidopsis database and allowing phosphorylation of Thr and Ser residues as possible modifications.
c
This peptide was detected with a score below the statistical limit for reliable identification.