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Tissue specificity for np-Ga3PDHase phosphorylation. A, Phosphorylation of np-Ga3PDHase by wheat endosperm extract. B, Phosphorylation of np-Ga3PDHase by wheat leaves extract. 1, Optimal phosphorylation conditions for WPK4. 2, Optimal phosphorylation conditions for SOS2. Left panels, Coomassie Brilliant Blue staining; right panels, [32P]γ-ATP incorporation in autoradiography of SDS-PAGE. Arrows indicate np-Ga3PDHase.
