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. 2011 May 5;156(3):1337–1350. doi: 10.1104/pp.111.177261

Figure 5.

Figure 5.

Divalent cation dependence of the wheat endosperm SnRK1 activity. Phosphorylation of np-Ga3PDHase by the SnRK1 partially purified from wheat endosperm was performed at various concentrations of Mg2+ (as MgCl2, gray squares) or Mn2+ (as MnCl2, black circles; A) and Ca2+ (as CaCl2) without Mg2+ (gray circles) or with 5 mm Mg2+ (black circles; B). Values of percentage of phosphorylation were calculated from autoradiography using LabImage Version 2.7.0 (free edition). Curves were made by triplicate and reproducible in ±10%.