Figure 7.

Effect of different metabolites on wheat endosperm SnRK1 activity. Assays of np-Ga3PDHase phosphorylation were performed in the presence of 2.5 mm Mg²⁺ and with addition of none (1), 2.5 mm ADPGlc (2), 5 mm ADPGlc (3), 2.5 mm Glc6P (4), 5 mm Glc6P (5), 2.5 mm Fru1,6bisP (6), 5 mm Fru1,6bisP (7), 0.5 mm Rib5P (8), 1.0 mm Rib5P (9), 2.5 mm Rib5P (10), 5 mm Rib5P (11), 2.5 mm 3PGA (12), or 5 mm 3PGA (13). The top plot was calculated from autoradiography (a) of SDS-PAGE gel stained with Coomassie Brilliant Blue (b). All data were reproduced (within ±10%) in three independent experiments.