Figure 3.

Silencing of the gfp gene in tomato fruits. A, Transverse sections of tomato fruits from GFP#5 transgenic plants, illuminated with a hand-held UV lamp, infiltrated in the inflorescence peduncle with MES buffer or pTRV1/pTRV2gfp Agrobacterium cultures. Fruits were sampled 45 d (MG) and 60 d (ripe) after infiltration. NS indicates nonsilenced fruit regions, while S indicates silenced regions. Bar = 1 cm. B, Expression analysis of the gfp and CP genes by qRT-PCR in MG and ripe tomatoes. Data indicate relative expression means from five replicates ± se. Asterisks indicate statistically significant differences (P < 0.05). All qRT-PCRs were performed in triplicate. [See online article for color version of this figure.]