Figure 1.

Effect of HSC70 and HSP90 deregulation on plant water losses in response to environmental conditions. A, Rate of fresh weight (FW) loss in darkness of 3- to 4-week-old detached rosettes from different Arabidopsis genotypes was measured after 195 min. Four plants per genotype were used and the experiment performed in triplicate. B, Bright-field (left) and thermal imaging (right) of 5-week-old plants: wild-type (Col), hsc70-3.1 (70-3.1), and ost2-2D (ost2) mutants and an HSC70-1 OE line (8.9). C and D, Leaf conductance was measured on attached leaves from 6-week-old plants at 22°C in darkness or light and ambient (400 μL L⁻¹) or high (2,000 μL L⁻¹) CO₂. C, Leaf conductance values were taken at the equilibrium while its speed of variation (D) was measured over a 10-min window following the change of condition. Three independent experiments were performed on at least three different plants. Error bar indicate standard deviations. * and **, Significant differences compared to the wild type (Student’s t test, P < 0.05 and P < 0.01, respectively). nd, Not determined.