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. 2011 May 17;156(3):1481–1492. doi: 10.1104/pp.111.174425

Figure 6.

Figure 6.

SGT1b, HSC70-1, and HSP90 deregulation causes hypersensitivity to ABA in seed germination assays. A, Fresh seeds harvested simultaneously from different genotypes were sown on Murashige and Skoog/10 in absence or presence of 5 μm ABA. Radicule emergence (germination) was scored after 5 d. The average germination rate is expressed as a percentage of the germination rate in absence of ABA for each genotype tested. * and **, Significant differences compared to the wild type (Student’s t test, P < 0.05 and P < 0.01, respectively). B, Fresh wild-type Col-0 seeds were sown on Murashige and Skoog/10 in absence or presence of 3 μm ABA in combination with 0 to 100 nm radicicol and germination scored as in A. The average germination rate is expressed as a percentage of the germination rate in absence of ABA and radicicol. * and **, Significant differences compared to the no radicicol control (Student’s t test, P < 0.05 and P < 0.01, respectively). Approximately 50 seeds per condition were scored in triplicate samples. Error bars indicate sds. Experiments were performed at least twice.