Figure 2.

RT-PCR gene expression analysis. The Pup1 genes indicated were analyzed by RT-PCR using RNA samples derived from roots and shoots of plants grown in P-deficient soil with (+P) and without (−P) P fertilizer application. Two different tolerant Nipponbare-Pup1 NILs with the Kasalath Pup1 locus were used (NIL24-4 = 1+; NIL14-4 = 2+). Nipponbare (NB) and a NIL14-4 sister line without the tolerant Pup1 locus (NIL14-6 = −) were used as intolerant controls. cDNA samples were analyzed by PCR using the cycle number indicated on the right. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was included as a control. H₂O, Water control.