Figure 3.

Analysis of cax1/cax3 mutants grown under various exogenous Ca²⁺ concentrations. The phenotypes (A) and P_i concentrations in the shoot (B) are shown for 21-d-old wild-type (WT) and cax1/cax3 plants grown under +P_i (250 μm KH₂PO₄) conditions supplemented with 0.05, 0.25, 1, or 2 mm CaCl₂. An additional 15 mm MgCl₂ was added to the medium containing 2 mm CaCl₂. Values represent means + sd of three biological replicates. Data significantly different from the corresponding wild-type controls are indicated (* P < 0.05, ** P < 0.01; Student’s t test). FW, Fresh weight.