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. 2011 May 16;156(3):1556–1564. doi: 10.1104/pp.111.178012

Figure 1.

Figure 1.

Infection of Arabidopsis by PVX 2.7. A, Symptoms induced in wild-type Arabidopsis (Col-0) by the PVX 2.7 strain. Three leaves per plant were rub inoculated with sap made from either PVX UK3-infected tobacco (PVX UK3) or PVX 2.7-infected N. clevelandii (PVX 2.7). Photographs were taken 3 weeks after inoculation. B, Representative immunoblots of PVX CP accumulation in Arabidopsis and N. benthamiana. Total protein extracts were prepared from inoculated Arabidopsis or N. benthamiana leaves 5 or 6 d, respectively, after inoculation, and subjected to SDS-PAGE followed by anti-CP immunoblotting. Coomassie (left) or Ponceau (right) staining of the same extracts is shown to demonstrate equal loading. Each lane corresponds to the pool of the three inoculated leaves from a single plant for Arabidopsis and a single leaf for N. benthamiana. Three plants per treatment were tested in each Arabidopsis experiment and the experiment was repeated five times for Arabidopsis and twice for N. benthamiana.