Figure 3.

Coinoculation of PVX and PepRSV on Arabidopsis. Three leaves per plant were rub inoculated with sap containing PVX UK3, sap containing PepRSV, or a mixture of both saps. A, Photographs were taken 3 weeks post infection. Enlargements of the singly or doubly infected rosettes are shown in the bottom section. B, Representative immunoblot analysis of the PVX CP accumulation in inoculated leaves. Total protein extracts were prepared from inoculated leaves 5 d after inoculation and subjected to SDS-PAGE followed by anti-CP immunoblotting. Coomassie staining of the same extracts is shown to demonstrate equal loading. Each lane corresponds to the pool of three inoculated leaves from the same plant. Three plants per treatment were tested in each experiment and the experiment was repeated five times.