Figure 5.

PVX infection of an Arabidopsis triple DICER mutant. A, Symptoms induced by PVX UK3 infection in wild type and dcl2 dcl3 dcl4 mutant Arabidopsis. Three leaves per plant were rub inoculated with sap containing PVX UK3. Photographs were taken 4 weeks after infection. B, Immunoblot analysis of PVX CP accumulation in inoculated leaves. Total protein extracts were prepared from inoculated leaves 6 d after inoculation and subjected to SDS-PAGE followed by anti-CP immunoblotting. Coomassie staining of the same extracts is shown to demonstrate equal loading. Each lane corresponds to a pool of the three inoculated leaves from a single plant. Four plants per treatment were tested in each experiment and the experiment was repeated five times.