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. 2011 May 16;156(3):1556–1564. doi: 10.1104/pp.111.178012

Figure 7.

Figure 7.

Systemic movement of PVX in Arabidopsis RNA-silencing mutants. A, Immunoblot analysis of PVX CP accumulation in Arabidopsis systemic tissue. Total protein extracts were prepared from the systemic leaves of one Arabidopsis plant of each genotype 3 weeks after PVX inoculation, followed by SDS-PAGE and anti-CP immunoblotting. Coomassie staining of the same extracts is shown to demonstrate equal loading. The triple dicer mutant sample is taken from a separate experiment but similar amounts of tissue and exposure times were used. B, Detection of PVX by inoculation of Rx2 transgenic N. benthamiana. Sap was prepared from the systemic tissue of PVX UK3-infected wild type and ago mutant Arabidopsis. The left section indicates a representative result (lack of HR lesions) obtained from inoculation with sap from wild type, ago1-27, or mutants in ago3 to ago10. The right section depicts a representative result showing HR lesions, indicating the presence of PVX, observed upon inoculation with sap preparations from individual ago2-1 plants.