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. 2011 May 11;156(3):1603–1611. doi: 10.1104/pp.111.177030

Figure 2.

Figure 2.

Subunit structure of Rubisco. A, Analysis of Rubisco by BN-PAGE. Total soluble proteins (1.0 μg for rice and 2.0 μg for sorghum) were separated on a 3% to 12% gradient gel and stained with Coomassie Blue. B, SDS-PAGE and immunoblotting of Rubisco separated by BN-PAGE. Rubisco bands from BN-PAGE were excised from the gel and separated by SDS-PAGE. Polypeptide bands were detected by Coomassie Blue staining (left section) and immunoblotting with an antiserum raised against the rice Rubisco (right section). NT, Nontransgenic rice; SS5, SS10, SS16, transgenic rice that express sorghum RbcS.