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. 2011 Apr 18;156(3):1164–1175. doi: 10.1104/pp.111.175240

Figure 2.

Figure 2.

Functional expression of OsPT8 in yeast and oocytes. A, Functional characterization of OsPT8 in a yeast mutant. a, Staining test for acid phosphatase activity in the yeast strain MB192 (control), Yp112-OsPT8, which contains OsPT8 in MB192, and the wild type (WT). The culture medium contains 0.02, 0.06, and 0.10 mm Pi, respectively. b, Growth curves of the wild type, MB192, and MB192 transformed with Yp112-OsPT8 generated from a 24-h culture under 60 μm Pi. c, Effects of different pH levels in the culture medium on the growth of the three yeast strains: Yp112-OsPT8, MB192, and the wild type. d, Velocity of 33Pi transport by Yp112-OsPT8 as a function of Pi concentration. The nonlinear regression of Pi uptake of strain Yp112-OsPT8 versus the external concentration at pH 6.5 was used to estimate the apparent Km value for Pi uptake. OD(600), Optical density at 600 nm. B, Functional characterization of OsPT8 in Xenopus oocytes. The absorption of 33Pi by oocytes injected with OsPT8 mRNA and water is shown.