Figure 3.

Expression of the wheat (A) and barley (B) PAPhy isogenes. Developing grains at 15, 21, and 35 DPA were dissected into three fractions, embryo (EM), endosperm (EN), and seed coat (SC), containing pericarp and aleurone. Germination grains were examined at 2, 4, and 6 d after germination (DAG) and were dissected into three fractions, early primary leaves (L), early primary root (R), and a fraction consisting of the germinated grain minus the primary leaf and root (S). Data represent averages of three biological repeats each in three technical repeats. Relative expression units have been transformed to expression fold (log₂) relative to α₂-tubulin expression (0-fold expression).