Table I. Specific phytase activity of purified r-TaPAPhy_a1 and b1 without and with bivalent metal ions.

The enzymes and metals were incubated for 10 min at room temperature before assaying for phytase activity.

Enzyme	No Metal	FeSO4 (10 mm) + Ascorbic Acid (3 mm)	FeCl3 (10 mm)	CaCl2 (10 mm)	MnSO4 (10 mm)
r-TaPAPhy_a1 activity (μmol phosphorus min−1 mg−1)	18.45 ± 1.49	27.65 ± 2.45	17.45 ± 1.56	19.34 ± 2.34	223.5 ± 4.68
Relative activity (%)	100	150	95	105	1,210
r-TaPAPhy_b1 activity (μmol phosphorus min−1 mg−1)	36.24 ± 2.12	216 ± 5.11	32.45 ± 3.56	35.45 ± 3.41	41.30 ± 4.78
Relative activity (%)	100	584	90	98	114