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. 2011 Jan 10;156(3):1087–1100. doi: 10.1104/pp.110.164756

Table I. Specific phytase activity of purified r-TaPAPhy_a1 and b1 without and with bivalent metal ions.

The enzymes and metals were incubated for 10 min at room temperature before assaying for phytase activity.

Enzyme No Metal FeSO4 (10 mm) + Ascorbic Acid (3 mm) FeCl3 (10 mm) CaCl2 (10 mm) MnSO4 (10 mm)
r-TaPAPhy_a1 activity (μmol phosphorus min−1 mg−1) 18.45 ± 1.49 27.65 ± 2.45 17.45 ± 1.56 19.34 ± 2.34 223.5 ± 4.68
Relative activity (%) 100 150 95 105 1,210
r-TaPAPhy_b1 activity (μmol phosphorus min−1 mg−1) 36.24 ± 2.12 216 ± 5.11 32.45 ± 3.56 35.45 ± 3.41 41.30 ± 4.78
Relative activity (%) 100 584 90 98 114