Figure 7.
NO levels in the wild-type Col-0 and Ler as well as in nia1,2noa1-2, phyB, ga1-3, and gai-1D mutant plants. A, Endogenous NO was visualized with the cell-permeable DAF-FM DA fluorescein in seedlings grown in darkness for 4 d and 1 h after the shift to red light (20 μmol m−2 s−1). B, NO-related fluorescence in Ler, ga1-3, and gai-1D in untreated (Control) and 50 μm GA3-treated seedlings for 2.5 h. Values are shown relative to untreated wild-type levels. The right panel of A and B show the quantification of three independent experiments as mean values of fluorescence (arbitrary units [a.u.]) ± se. Asterisks represent statistically significant differential values at P < 0.05 when comparing controls in darkness (A) or untreated (B).