Pi uptake and remobilization analysis in wild-type and ltn1 mutant plants. A, [32P]Pi uptake activity in roots of wild-type (WT; black diamonds) and ltn1 mutant (gray squares) plants. FW, Fresh weight. Error bars indicate sd (n = 3). B, Shoot-to-root ratios of the [32P]Pi taken up by wild-type (black bars) and ltn1 mutant (gray bars) plants from A. Error bars indicate sd (n = 3). C, Autoradiograph of [32P]Pi taken up by wild-type and ltn1 mutant plants. D and E, Pi concentration alteration in the fourth leaf (D) or roots (E) of wild-type (black diamonds) and ltn1 mutant (gray squares) plants after Pi cutoff. Error bars indicate sd (n = 5).