Figure 9.

Nitrate contents and nitrate-related gene expression analysis in wild-type and ltn1 mutant plants. A, Nitrate concentration in roots (black bars) and shoots (gray bars) of wild-type plants under Pi-sufficient (HP; 16 mg L⁻¹) or Pi-deficient (NP; 0 mg L⁻¹) conditions. Error bars indicate sd (n = 3). DW, Dry weight. Asterisks indicate the significance of differences between wild-type plants grown under HP and NP conditions as determined by Student’s t test analysis: ** P < 0.01. B, Nitrate concentration in roots (black bars) and shoots (gray bars) of wild-type (WT) and ltn1 mutant plants under Pi-sufficient conditions (16 mg L⁻¹). Error bars indicate sd (n = 3). Asterisks indicate the significance of differences between wild-type and ltn1 mutant plants as determined by Student’s t test analysis: * 0.01 ≤ P < 0.05, ** P < 0.01. C, qRT-PCR expression analysis of nitrate transporter (NT) and nitrate reductase (NR) genes in roots of wild-type (black bars) and ltn1 mutant (gray bars) plants under Pi-sufficient conditions (16 mg L⁻¹). Error bars indicate sd (n = 3). Asterisks indicate the significance of differences between wild-type and ltn1 mutant plants as determined by Student’s t test analysis: ** P < 0.01.