Table 3.
Mutagenic activity expressed as the mean and SD of the number of revertants/plate in bacterial strain TA98 exposed to the compounds isolated from methanolic extract of I. truxillensis and I. suffruticosa at various doses, with (+S9) or without (−S9) metabolic activation.
| Treatment (mg/plate) | TA98 (−S9) | TA98 (+S9) |
|---|---|---|
| Positive control | 867 ± 1.4 | 991 ± 10.1 |
| Negative control | 30 ± 1.5 | 36 ± 4.0 |
| Alkaloids | ||
| Indigo | ||
| 0.125 | 38 ± 0.6 (1.3) | 44 ± 2.7* (1.2) |
| 0.25 | 42 ± 3.2 (1.4) | 86 ± 5.3** (2.4) |
| 0.50 | 45 ± 1.5 (1.5) | 192 ± 2.5** (5.3) |
| 0.75 | 50 ± 4.0* (1.7) | 229 ± 3.6** (6.7) |
| 1.00 | 57 ± 4.2** (1.9) | 276 ± 5.3** (7.7) |
| Indirubin | ||
| 0.125 | 46 ± 2.7 (1.5) | 36 ± 2.0 (1.0) |
| 0.25 | 58 ± 3.8** (1.9) | 36 ± 4.0 (1.0) |
| 0.50 | 58 ± 3.0** (1.9) | 38 ± 2.5 (1.1) |
| 0.75 | 63 ± 1.7** (2.1) | 43 ± 4.0 (1.2) |
| 1.00 | 63 ± 3.6** (2.1) | 46 ± 3.6 (1.3) |
Negative control: DMSO (100 μl/plate); Positive control: −S9: 4-nitro-o-phenylenediamine (10.0 μg/plate); +S9: 2-anthramine (1.25 μg/plate).
*P < .05, **P < .01 (ANOVA). The values in brackets are MI values.