Skip to main content
. 2009 Jul 1;90(3):822S–825S. doi: 10.3945/ajcn.2009.27462T

FIGURE 2.

FIGURE 2

Secretion of glucagon-like peptide 1 (GLP-1) (A) and insulin (B) in response to glucose gavage (5 g/kg body weight) in α-gustducin knockout (α-gust−/−) mice and wild-type (α-gust+/+) mice. (C) Postfasting plasma glucose concentrations after feeding standard rodent diet in α-gust−/− mice and α-gust+/+ mice. (D) Secretion of GLP-1 in response to 10% glucose injected into surgically isolated duodenum in α-gust−/− mice, T1r3 knockout (T1r3−/−) mice, and α-gust+/+ mice. The duodenum was ligated away from the stomach and the rest of the intestines, and circulatory contact was maintained. (E) Secretion of GLP-1 ex vivo from minced proximal duodenum from α-gust−/− and α-gust+/+ mice in response to the addition of 10% glucose to the culture medium. (F) Secretion of GLP-1 ex vivo from isolated duodenal villi from α-gust−/− and α-gust+/+ mice in response to the addition of 10% glucose to the culture medium. For in vivo experiments, n = 5–12 animals/genotype; in vitro experiments were carried out in triplicate and replicated at least twice. All values are means ± SEMs. *,**,*** Statistical significance was determined by ANOVA: *P < 0.05, **P < 0.01, ***P < 0.001. Data are modified with permission from Reference 16.