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. Author manuscript; available in PMC: 2011 Jul 14.
Published in final edited form as: J Microbiol. 2010 Jun 23;48(3):378–386. doi: 10.1007/s12275-010-0022-0

Figure 4.

Figure 4

Anti-Fur Western blot. Purified recombinant H. pylori G27 Fur (rFur), and equal concentrations of lysates from Δfur H. pylori (Δfur) and Δfur H. pylori carrying the Fur constructs from C. jejuni (Cj), D. vulgaris (Dv), E. coli (Ec), H. pylori (Hp), P. aeruginosa (Pa), and V. cholerae (Vc) were subjected to Western blot analysis. (A) Martha 2472 rabbit polyclonal anti-P. aeruginosa Fur antibody was used to detect E. coli, P. aeruginosa, and V. cholerae Fur. (B) Polyclonal rabbit anti-C. jejuni Fur antibody was used to detect C. jejuni, E. coli, P. aeruginosa, and V. cholerae Fur proteins. (C) Polyclonal rabbit anti-H. pylori Fur antibody was used to detect recombinant H. pylori Fur, C. jejuni, D. vulgaris, E. coli, H. pylori and P. aeruginosa Fur. These data are representative of multiple independent experiments.