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. Author manuscript; available in PMC: 2011 Jul 14.
Published in final edited form as: Mol Cell Neurosci. 2006 Nov 20;34(1):59–68. doi: 10.1016/j.mcn.2006.10.003

Figure 1. Identification of MSC markers and multilineage potential by differentiation to known cell types.

Figure 1

A. Bone marrow MSCs from passage 3 were immunostained with antibodies against CD44, CD45, CD34 and Sca-1 followed by secondary antibodies against mouse immunoglobulins labeled with TRITC (shown in red). Staining for CD34 and CD45 was negative, but CD44 and Sca-1 were expressed. Nuclei were stained with DAPI (blue). B. Immunolabeling for CD44 and nestin (shown in green). The merged image on the right shows co-staining of a population of cells with both markers. C. Co-expression of nestin and Sca-1. Merged images show co-staining. D. Analysis of bone marrow MSCs by chip flow cytometry indicates the ratio of immunopositive cells for each of these antibodies. E. Potential for lineage differentiation was shown by formation of chondrocytes and extracellular matrix after treatment of bone marrow MSCs with TGF-β. The cells that grew out from a micro-aggregate (top) were stained for type II collagen (bottom). F. Demonstration of lineage potential of bone marrow MSCs to neurons by differentiation in serum-free medium containing neuronal growth supplements and bFGF. Staining for neurofilament (NF-M) is shown in these cells.