. Author manuscript; available in PMC: 2011 Jul 14.

Published in final edited form as: Chembiochem. 2007 Apr 16;8(6):632–641. doi: 10.1002/cbic.200600528

Table 2.

Strains used in this study

Strain	Relevant genotype/comments	Source/ref.
E. coli
DH10B	F⁻ mcrA Δ (mrr- hsdRMS-mcrBC) ϕ80dlacZ ΔM15 ΔlacX74 deoR recA1 endA1 ara Δ139 D(ara,leu)1697 galU galK λ⁻rspL nupG	GibcoBRL
ET12567(pUZ8002)	dam dcm hsdS, pUZ8002	^[24]
XL-1Blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac	Stratagene
BL-21 Gold(DE3)/pLysS	F⁻ ompT hsdSB (r_B⁻ m_B⁻) gal dcm (DE3) pLysS (Cm^r)	^[25]
S. hygroscopicus
5008	Wild-type producer of validamycin
ZYR-1	Non-producer of validamycin generated by replacing a 913-bp DNA fragment internal to valC	This work
ZYR-1/pJTU704	ZYR complemented with pJTU704 harboring full-length valC	This work
Pellicularia sasakii	Fungal indicator strain sensitive to validamycin A	SJUSC