Figure 10.

Effects of peroxidized EPA/DHA on the phosphorylation and degradation of IκB proteins. Cells were treated with IL-1β (1 nM) in the presence or absence of peroxidized EPA for 3 days and DHA for 1.5 days (each, 500 μM) for the indicated times. (a) Phosphorylation and degradation of IκBα, and (b) degradation and recovery of IκBα and IκBβ. Cell lysates (25 μg of protein) were subjected to SDS-PAGE in a 12.5% gel, followed by immunoblotting with an anti-phospho-IκBα, anti-IκBα or anti-IκBβ antibody.