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Effects of peroxidized EPA/DHA on the expression of iNOS mRNA. Cells were treated with IL-1β (1 nM) in the presence or absence of peroxidized EPA for 3 days and DHA for 1.5 days (each, 500 μM) for the indicated times. Total RNA was analyzed by strand-specific RT-PCR to detect iNOS mRNA, using EF mRNA as an internal control.
