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FP ssDNA binding assays for RPA-CDE-core, RPA-DE and RPA-C at 10 mM NaCl. Representative binding isotherms are shown. (a)–(d) RPA-CDE-core, (e)–(h) RPA-DE, and (i)–(l) RPA-C. The layout of the figure is the same as described in Figure 4. All experiments were performed at a lowered NaCl concentration to observe binding by the low-affinity RPA constructs and for comparison with RPA-CDE-core.
