Skip to main content
. 2011 Jun 27;108(28):11584–11589. doi: 10.1073/pnas.1018726108

Fig. 3.

Fig. 3.

Construction of synthetic switch region. (A) Structure of the 80-bp linker (monomer) reflecting the consensus Sα repeat described in ref. 47. (B) Resolution of ligated concatemers on a 1.2% agarose gel. Lane 1, 1-kb DNA ladder (Invitrogen). Lane 2, ligation products (concatemers). The number of repeats in each band was shown to the right side of the gel. (C) Comparison of class-switch efficiency between the size-matched synthetic and native core Sα region. Each dot represents an independent clone. One of at least three independent experiments was shown. (D) The level of GLTs in stimulated cells. Error bars represent SEM of at least three independent experiments. Each experiment contains at least two independent clones from each construct.