Fig. 2.

Suppressive effect of ISM ODN on nucleic acid-mediated innate immune responses. (A and B) MEFs were left untreated (control) or pretreated with the indicated ODN (1 μM) for 1 h, and then stimulated with B-DNA (5 μg/mL) or poly(I:C) (5 μg/mL) for 3 h (A and B) or 6 h (A). (C) Bone marrow-derived pDCs were left untreated (control) or pretreated with ISM ODN for 1 h, and then stimulated with CpG-A ODN (1 μM) or poly(U) (5 μg/mL) for 6 or 12 h. Cytokine mRNA expression levels of the indicated genes were measured by quantitative RT-PCR. *P < 0.01 compared with control. Data in all panels are presented as means and SD (n = 3). ND, not detected.