Fig. 2.

qRT-PCR analysis of the abundances of gene transcripts in the oro mutant compared with wild-type gametophyte (GA) and partheno-sporophyte (pSP). The abundance of transcripts of four genes that have been shown to be differentially expressed in the gametophyte and sporophyte generations (18) was assayed in oro individuals. Data are means of three independent biological replicates ± SE. Transcript abundance was significantly different in the oro and GA samples compared with the wild-type pSP sample for all of the genes tested (ANOVA, P < 0.05). (From top to bottom) IDW6, Esi0351_0007; IDW4, Esi0085_0048; IUP6, Esi0031_0170; IUP2, Esi0416_0001.