Fig. 4.

Phylogenetic analysis of conserved regions in the helicase (motifs I–VI) (A) and RdRp (B) genes of CHV aligned with representative members of the Hepacivirus, Pegivirus (GBV viruses A, C, and D), Pestivirus, and Flavivirus genera. Translated amino acid sequences were aligned with the program ClustalW. Trees were constructed by neighbor joining of pairwise amino acid distances with the program MEGA5 (according to the distance scale provided). Bootstrap resampling was used to determine robustness of branches; values of ≥70% (from 1,000 replicates) are shown. Regions compared corresponded to positions 3697–4477 (helicase domain of NS3) and 7705–8550 (RdRp in NS5B; numbered according to the AF011751 HCV genotype 1a reference sequence). A listing of virus abbreviations and original accession numbers for each sequence are provided in Table S2.