Figure 4.

A) Schematic demonstrating components and orientation of photomicrographs. B) Confocal microscopic images of TEVG implanted into female host that had undergone transplantation with male bone marrow harvested 6 mo after implantation, demonstrating colocalization of macrophages (MAC) and the Y-chromosome (Y-chromosome FISH) but no colocalization with the Y chromosome (Y-chromosome FISH) and either endothelial cells (vWF) or smooth muscle cells (Calponin; ×400). C) Numbers of infiltrating BM-derived Y chromosome (Y-chromosome FISH) cells (bone marrow cells/high power field) in the graft wall increased up to 10 wk, while graft degradation was occurring, but at 6 mo postimplantation, these cells markedly decreased. D) This was quantified and corroborated using qRT-PCR for GFP DNA within the graft over a 6-mo period. E) Confocal microscopic image of triple staining of a 6-mo neovessel with normal IVC configuration with single-layer neointima (EC; vWF/light blue), neomedia (SMC; calponin/green), and neoadventitia with scattered inflammatory cells (male; Ychr/red).