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. 2011 Aug;25(8):2604–2614. doi: 10.1096/fj.10-175802

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Figure 1. — Analysis of rabbit spermatogonia. A) Histological examination of a 15-wk-old prepubertal rabbit testis. Left panel: no spermatids were found in the seminiferous tubules. Right panel: enlarged image of the boxed area in left panel. Arrows indicate spermatogonia. Scale bars = 50 μm (left panel); 15 μm (right panel). B) Flow cytometric analysis of testicular cells isolated from 15-wk-old rabbit testes. Gate 1 (G1) and gate 2 (G2) were created by analysis of forward scatter (FS) and side scatter (SS). Cells in G1 were FS^lo ITGA6⁻; cells in G2 were FS^hi ITGA6⁺. C) Flow cytometric analysis of G2 cells of panel B. FS^hi ITGA6⁺ cells expressed THY1, CD9, and GFRA1. Proportions of ITGA6⁺ THY1⁺, ITGA6⁺ CD9⁺, and ITGA6⁺ GFRA1⁺ cell fractions in the total cells analyzed were 25, 11, and 17%, respectively. The x and y axes depict the relative fluorescence intensity of individual cells.