Figure 2.
Malignant tumors down-regulate NGP expression in myeloid cells and in MDSCs from bone marrow or tumor tissues. A) 32D cells were incubated with conditioned medium collected from 67NR or 4T1 cells or with growth medium (GM) for 24 h. NGP transcript levels were analyzed by real-time PCR. Data represent average ± sem ΔΔCT values compared to untreated cells. Each experiment was completed in triplicate and repeated twice. Results are significant by 1-tailed t test as indicated by P values. B, C) Gr1/CD11b cells were isolated from bone marrow (B) and tumor tissues (C) of mice bearing similar size 67NR tumors or 4T1 tumors by magnetic sorting (n=6 mice/group). Data represent average ± sem ΔΔCT values compared to MDSCs derived from 67NR tumor-bearing animals. RNA was isolated for real-time PCR to determine NGP expression. Experiments were repeated twice. All data were significant by 2-tailed paired t test, P < 0.05.