Figure 3.
PrPSc tissue uptake. Mice received [131I]PrPSc (1×106 cpm; 11.8 ng) and [125I]albumin (5×105 cpm) intravenously and were then killed 1 to 180 min after the injection. Brain (A), spleen (B), kidney (C), liver (D) skeletal muscle (E), heart (F), and lung (G) tissues were collected immediately. Cumulative excretion of degraded PrPSc in urine (H) was measured by collecting urine directly from the bladder. Points represent means ± se of 3–5 mice. Two-way ANOVA revealed that time curves of [131I]PrPSc and [125I]albumin differed significantly over the study period (A–G). There was no statistical difference in urinary excretion rate (H).