Figure 5.

Elevated ERO1α expression in the overloaded heart. Top panels: immunoblot of ERO1α in extracts of heart from wild-type (WT; lanes 1–7) or Ero1α mutant mice (lane 8) obtained 8 wk after a sham operation (S) or a TAC procedure (T). ERp57, an ER marker protein, and eIF2α, a cytosolic marker protein, serve as loading controls. Lysate of spleen from a wild-type animal (Sp), a rich source of ERO1α, was applied to lane 9 to clearly demarcate its position on the blot. Note the absence of ERO1α signal from the mutant heart in lane 8. Asterisk marks an irrelevant protein detected by the ERO1α antiserum in heart lysates. Bottom panel: relative levels of ERO1α protein, normalized to the ER loading marker. Values are means ± se. **P < 0.01.