Table 5.
Expression fold change of selected genes confirmed by RT-PCR
| Gene | Name | Hyp | Dmp1−/− | Hyp/Dmp1−/− |
|---|---|---|---|---|
| Phex | Phosphate regulating endopeptidase homolog, X-linked | NA | 1.1 ± 0.04 | NA |
| Dmp1 | Dentin matrix protein 1 | 1.6 ± 0.1 | NA | NA |
| Fgf23 | Fibroblast growth factor 23 | 372.0 ± 44.1* | 318.3 ± 81.6* | 311.9 ± 95.3* |
| Fcrls | Fc receptor-like S, scavenger receptor | 14.2 ± 1.0* | 9.1 ± 1.5*,# | 12.0 ± 1.9* |
| Lipg | Lipase, endothelial | 7.3 ± 1.1* | 7.7 ± 1.9* | 6.2 ± 1.6* |
| Ctsw | Cathepsin W | 5.1 ± 0.2* | 2.8 ± 0.1*,# | 4.3 ± 0.01*,$ |
| Fgf1 | Fibroblast growth factor 1 | 3.5 ± 0.07* | 3.9 ± 0.3* | 3.1 ± 0.2* |
| Csdc2 | Cold shock domain containing C2, RNA binding | 2.6 ± 0.3* | 2.1 ± 0.7* | 2.7 ± 0.5* |
| S100b | S100 protein, β polypeptide, neural | 2.9 ± 0.1* | 2.0 ± 0.04*,# | 2.1 ± 0.1* |
| Add3 | Adducin 3 (γ) | 1.3 ± 0.05* | 1.8 ± 0.1* | 1.9 ± 0.05*,# |
Values are expressed as fold change compared to the WT value; n = 3 samples/group.
*
P < 0.05 vs. WT;
#
P < 0.05 vs. Hyp;
$
P < 0.05 vs. Dmp1−/−; 1-way ANOVA and post hoc Fisher test.