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The time-of-addition effect of excoecarianin against HSV-2 infection. Excoecarianin added prior to virus infection and then washed out did not protect the cells from HSV-2 infection. The addition of excoecarianin after 2 h of the virus inoculation also did not inhibit HSV-2 infection. The compound can inhibit HSV-2 infection only when it was added concurrently with the virus infection.
