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Chemical standardization of DBT by HPLC fingerprint analysis. In the HPLC fingerprint at an absorbance of 254 nm, the peaks corresponding to ferulic acid, calycosin, formononetin, and ligustilide in DBT, RA, and RAS are indicated by arrowheads. The details of the marker identification were described previously [8]. These chemical characterizations are used to identify the standardized extracts for all the biochemical analyses. Typical fingerprints are shown.
