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. Author manuscript; available in PMC: 2012 Jul 14.
Published in final edited form as: J Med Chem. 2011 Jun 8;54(13):4923–4927. doi: 10.1021/jm200304y

Figure 3.

Figure 3

Effects of fatostatin (1) and analog 24 on the proteolytic activation of SREBP-2 in cells. Top: western blot analysis of SREBP-2. CHO-K1 cells were treated with 1% (v/v) DMSO (vehicle), varied concentrations (1, 5, and 20 μM) of fatostatin (1) or compound 24, or sterols (10 μg mL−1 cholesterol or 1 μg mL−1 25- hydroxycholesterol). The precursor and mature forms of SREBP-2 were detected by an SREBP-2 antibody. The positions of the precursor and mature forms of SREBP- 2 are indicated. Bottom: quantification of the western blot analysis. Percentages of the mature form of SREBP-2 are shown. Values are means ± S.D. of three independent experiments.