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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Proteome Res. 2011 May 31;10(7):3149–3159. doi: 10.1021/pr200210w

Figure 3. Manual validation of uniquely expressed proteins.

Figure 3

Peptides that were detected in one member of a SILAC pair but not the other were manually validated for absence of the corresponding peptide. A) Sample spectra show peaks mapping to the QLLLSAALSAGK peptide of chitinase-3-like protein 1 in both forward and reverse SILAC experiments, indicating the absence of the peptide in the T98 conditioned media. B) Census quantitation of the same peptide with forward and reverse labeling.