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. Author manuscript; available in PMC: 2011 Nov 2.
Published in final edited form as: Biochemistry. 2010 Nov 2;49(43):9190–9198. doi: 10.1021/bi1013722

Figure 3. 13C 1D and 1H-13C 2D spectra of POPC bilayer in a Nanodisc.

Figure 3

(a) 1D 13C spectrum of Nanodiscs with uniformly-13C,15N-labeled MSP1D1 and natural abundance POPC lipids at 8 °C on a 600 MHz (1H frequency) spectrometer. The lipid spectrum was acquired with a 1 ms T2 filter, no 1H-1H mixing and 0.4 ms 1H-13C contact time. The data was processed with 25 Hz Lorentzian-to-Gaussian line-broadening. (b) 1H-13C 2D spectrum of the same sample acquired under the same conditions for 6.5 hrs, with a schematic of a POPC lipid molecule. The spectrum was processed with 100 Hz of Lorentzian-to-Gaussian line broadening in each dimension.