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. Author manuscript; available in PMC: 2011 Nov 2.
Published in final edited form as: Biochemistry. 2010 Nov 2;49(43):9190–9198. doi: 10.1021/bi1013722

Figure 4. 13C 1D spectra of MSP1D1 in POPC Nanodiscs.

Figure 4

Spectra were acquired on U-13C,15N-labeled MSP1D1 at 8 °C. 13C 1D spectra were acquired with a 1 ms T2 filer, a 0.4 ms HC contact time, where 1H-1H mixing time was incremented and sequentially set to 3 μs, 1 ms, 3 ms, 10 ms and 30 ms. The data were processed with 30 Hz Lorentzian-to-Gaussian line broadening.