Table 5.
Fragment ions of the 28SAPSTGGVKKPHR40 peptide from histone H3 isolated from exponential phase Saccharomyces cerevisiae.
| Order | b ion (m/z) | b2+ ion (m/z) | Sequence | y ion (m/z) | y2+ ion (m/z) | Order |
|---|---|---|---|---|---|---|
| 1 | 88.0393 | 44.5233 | S | 13 | ||
| 2 | 159.0764 | 80.0418 | A | 1276.7120 | 638.8597 | 12 |
| 3 | 256.1292 | 128.5682 | P | 1205.6749 | 603.3411* | 11 |
| 4 | 343.1612 | 172.0842 | S | 1108.6222 | 554.8147* | 10 |
| 5 | 444.2089 | 222.6081 | T | 1021.5901 | 511.2987* | 9 |
| 6 | 501.2304 | 251.1188 | G | 920.5425 | 460.7749* | 8 |
| 7 | 558.2518 | 279.6295 | G | 863.5210 | 432.2641 | 7 |
| 8 | 657.3202 | 329.1638 | V | 806.4995 | 403.7534* | 6 |
| 9 | 827.4258 | 414.2165 | K | 707.4311 | 354.2192 | 5 |
| 10 | 955.5207 | 478.2640 | K | 537.3256* | 269.1664 | 4 |
| 11 | 1052.5735 | 526.7904 | P | 409.2306* | 205.1190 | 3 |
| 12 | 1189.6324 | 595.3198* | H | 312.1779 | 156.5926 | 2 |
| 13 | R | 175.1190 | 88.0631 | 1 |
The list of product ions observed (underlined), matched with Mascot to the SWISS-PROT database (asterisk superscript), or predicted following CID fragmentation is shown.