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ZA prevents RhoA membrane localization in LM8 cells. Treatment of LM8 cells with ZA (10 μM) or clodronic acid (10 μM) for 24 hours (A). In the presence or absence of GGOH(20 μM) or FOH(20 μM) for 48 hours(A). Cells were extracted and separated intocytosolic and membrane fractions to detect RhoA by immunoblotting as described in the experimental section. These results are representative of three independent experiments.
