Figure 1.

Characterization of [³H]-neurosteroids released in the incubation medium by: (a) lumbar SC slices after a 3-h incubation with [³H]PROG in the absence (b) or in presence of gelsemine 5 cH or (c) Gelsemium sempervirens 5 cH. Analyses were performed using a hexane/isopropanol gradient and a reverse-HPLC system coupled to a flow scintillation detector. The ordinate indicates the radioactivity measured in the HPLC eluent. The dashed line represents the gradient of secondary solvent (% isopropanol). The arrows indicate elution positions of standard steroids.