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. 2011 Jul 14;7(7):e1002172. doi: 10.1371/journal.pgen.1002172

Figure 5. Adverse ABA phenotypes of transgenic plants overexpressing HB33 and reducing HB33 by RNAi.

Figure 5

(A) The relative expression of HB33 in different transgenic lines. The wild type (WT) was used as a standard control. OE10 and OE16 were selected for further study. (B,C) Comparison of seed germination greening among WT, arf2-101, HB33 overexpression line OE10 and OE16 on MS medium supplemented with 0, 0.3 and 0.5 µM ABA. Three independent experiments were done. Values are means ±SD, **p<0.01. (D,E) Comparison of relative root growth among WT, arf2-101, OE10 and OE16. Five-day-old seedlings grown on MS medium were transferred to MS medium containing 0, 10, 30 and 50 µM ABA and were grown for 7 days before being photographed and statistics analysis. The dot line in (D) indicates the places where the root tips were after just transferring. 30 seedlings were measured in each experiment. Relative root growth represents the root growth of seedlings after treatment with ABA comparing with that without ABA treatment. Three independent experiments were done. Values are means ±SD, **p<0.01. (F) The relative expression of HB33 in two RNAi transgenic lines (hb33-1 and hb33-2). WT was used as a standard control. (G,H) Comparison of seed germination greening among WT, arf2-101, hb33-1 and hb33-2 on MS medium supplemented with 0, 0.3 and 0.5 µM ABA. Three independent experiments were done. Values are means ±SD, **p<0.01. (I,J) Comparison of relative root growth among WT, arf2-101, hb33-1 and hb33-2. Five-day-old seedlings grown on MS medium were transferred to MS medium containing 0, 10, and 30 µM ABA and were grown for 7 days before being photographed and statistics analysis. The dot line indicates the places where the root tips were after just transferring. Relative root growth represents the root growth of seedlings after treatment with ABA comparing with that without ABA treatment. 30 seedlings were measured in each experiment. Three independent experiments were done. Values are means ±SD, **p<0.01.