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. Author manuscript; available in PMC: 2011 Jul 14.
Published in final edited form as: Chembiochem. 2009 Jan 26;10(2):304–314. doi: 10.1002/cbic.200800527

Figure 4.

Figure 4

(A) Partial TLC of reaction products of CetB single mutants. 1, 5-epi-valiolone standard; 2, 2-epi-5-epi-valiolone standard; 3, CetA reaction only; 4, CetA+CetB; 5-8, CetA+CetB single mutants: 5, H14G; 6, H99G; 7, E76G; 8, E151G. (B) Partial TLC of reaction products of CetB double mutants. 1, 5-epi-valiolone standard; 2, 2-epi-5-epi-valiolone standard; 3, CetA reaction only; 4, CetA+CetB; 5, CetA+H14G/H99G CetB with 1,10-phenanthroline; 6-11, CetA+CetB double mutants without 1,10-phenanthroline: 6, H14G/H99G; 7, E76G/E151G; 8, H14G/E76G; 9, H14G/E151G; 10, H99G/E76G; 11, H99G/E151G. (C) Partial TLC of reaction products of CetB mutants with 1,10-phenanthroline. 1, 2-epi-5-epi-valiolone standard; 2, 5-epi-valiolone standard; 3, CetA reaction only; 4, CetA+CetB without 1,10-phenanthroline; 5, CetA+CetB with 1,10-phenanthroline; 6-15, CetA+CetB mutants with 1,10-phenanthroline: 6, H14G; 7, H99G; 8, E76G; 9, E151G; 10, H14G/H99G; 11, E76G/E151G; 12, H14G/E76G; 13, H14G/E151G; 14, H99G/E76G; 15, H99G/E151G. (D) Partial TLC of reaction products of H99G CetB with different metal ions. 1, 2-epi-5-epi-valiolone standard; 2-9, CetA+H99G CetB with 1,10-phenanthroline (1 mM) and then metal ions (1.5 mM each) or water (negative control) were added: 2, Fe2+; 3, Co2+; 4, Ca2+; 5, Mg2+; 6, Ni2+; 7, Zn2+; 8, Cu2+; 9, Mn2+; 10, water. Stars indicate the production of 5-epi-valiolone.