Figure 4. NSP4 activates phospholipase C mediated IP₃ but not the cAMP pathway.

(A) GOT1 cells were stimulated with secretory and recombinant NSP4 (2 µM) for 60 min during accumulation of IP-one. Carbachol were used as positive control. Supernatants were then analysed for the IP₃ metabolite IP-one, using a commercial ELISA (n = 3). (B) GOT1 cells were stimulated with secretory or recombinant NSP4 (2 µM) for 5 min, with or without AC inhibitor, and cAMP was measured in cell lysates of stimulated cells (n = 3) by ELISA. Isoprotenerol (Isop.) were used as positive control.